# DRAGEN Heme WGS Tumor Only Pipeline

## Overview

DRAGEN Heme WGS Tumor Only Pipeline, henceforth referred as the Heme Pipeline, is a comprehensive and unbiased whole genome sequencing solution to replace conventional cytogenetic and panel sequencing approaches for detecting all types of mutation using a limited amount of DNA. It can be applied to detect clinically actionable mutations for cancer spanning a wide range of genomic events, e.g., structural variants (SV), Copy Number Alterations (CNA), small variants (SNV/insertion/deletion/delins) and internal tandem duplications (ITD) and DUX4 variants using Heme samples.

The Heme pipeline includes a DNA-only workflow designed to analyze whole genome sequencing data generated on supported instruments. It may be run as a local off-instrument solution installable on a DRAGEN server or accessible through the Illumina Connected Analytics (ICA) cloud environment. The Heme pipeline is for Research Use Only (RUO).

![Figure 1. DRAGEN Heme WGS Tumor Only Workflow](https://3555446211-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F-MflHs6hPlQAu-m5s2h_%2Fuploads%2Fgit-blob-a3b9e53093d0e4ef6180d4c89110869883ecede9%2Fdna-to-workflow.png?alt=media)

## Features

* Superb performance based on the DRAGEN BioIT platform Release 4.4.4
* Supports starting the analysis from BCL, FASTQ, BAM or CRAM as inputs.
* Flexible custom configurable options on top of well established DRAGEN recipes for Heme WGS analysis.
* Available on local DRAGEN servers and Illumina Connected Analytics (ICA)
* Seamless integration with Illumina Connected Insights (ICI) for tertiary interpretation

## Supported Library Prep Kits (LPKs)

* Illumina DNA PCR Free Prep Kit
* Illumina DNA Prep Kit
* Custom LPKs

## Supported Sequencing Instruments

* NovaSeq 6000 or 6000Dx in RUO mode
* NovaSeq X or NovaSeq X plus

> **Note** Unsupported instruments can still be analyzed, but a warning will be generated.

## Supported FLow Cells

* NovaSeq 6000 or 6000Dx S4
* NovaSeq X or NovaSeq X plus 10B, 25B