# Templates

## Description

Sample Sheet templates for the Heme pipeline for standalone DRAGEN server and ICA manual launch analysis can be found in the table below. For auto-launch compatible sample sheets, use BaseSpace Run Planner.

The Heme pipeline is compatible with several instruments and assay workflows (standard, XP), each of which have implications for the sample sheet.

## Templates

Sample sheet templates contain all required fields, including index sequences in the proper orientation for all indexes from a given library prep kit. The templates are provided as a starting point for creating a sample sheet manually when launching analysis on a standalone DRAGEN server or on ICA using manual launch.

For interactive run planning or to create a sample sheet for ICA Autolaunch, use [BaseSpace Run Planner](https://basespace.illumina.com/) to create valid sample sheets for either local or cloud analysis. To set up a run in BaseSpace run planner, refer to [Sample Sheet Creation in BaseSpace Run Planner](https://help.dragen.illumina.com/product-guides/dragen-v4.5/dragen-apps/dragen-apps/common/run-planning/basespace-run-planning-tool).

Users can visit the [Sample Sheet guidelines](https://help.dragen.illumina.com/product-guides/dragen-v4.5/dragen-apps/dragen-apps/dragen-heme-wgs-to-pipeline/quick-start/sample-sheet-requirements) section to learn additional details on required fields and values as they fill-in their sample information. Use the lookup table below to select and download the sample sheet template that matches your instrument, assay, and workflow configuration:

<table><thead><tr><th>Instrument</th><th>Workflow</th><th>Description</th><th data-type="files"></th></tr></thead><tbody><tr><td>NovaSeq 6000Dx (RUO)</td><td>Standard or XP</td><td>Cloud analysis in ICA with auto-launch generated with the BSSH run planner; compatible with analysis on local DRAGEN sever</td><td><a href="https://25033470-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FG9szlFZupV6Q2DasL98y%2Fuploads%2Fgit-blob-ce93af7ae8355f5fdffd89225d55d5fcd3783e0d%2FSampleSheet-NovaSeq6000-Cloud.csv?alt=media">SampleSheet-NovaSeq6000-Cloud.csv</a></td></tr><tr><td>NovaSeq 6000</td><td></td><td>Local Analysis on DRAGEN server; compatible with cloud analysis in ICA without auto-launch</td><td><a href="https://25033470-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FG9szlFZupV6Q2DasL98y%2Fuploads%2Fgit-blob-166c1e9442d8c9c6928da63d7e552354ecd49207%2FSampleSheet-NovaSeq6000-Local.csv?alt=media">SampleSheet-NovaSeq6000-Local.csv</a></td></tr><tr><td>NovaSeq X</td><td>Standard or XP</td><td>Cloud analysis in ICA with auto-launch of multiple pipelines for data from a mixed flow cell; compatible with analysis on local DRAGEN sever</td><td><a href="https://25033470-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FG9szlFZupV6Q2DasL98y%2Fuploads%2Fgit-blob-dff2b4c34986655cdc32db7af7ca65642e6cc25c%2FSampleSheet-NovaSeqX-Cloud-multi.csv?alt=media">SampleSheet-NovaSeqX-Cloud-multi.csv</a></td></tr><tr><td></td><td></td><td>Cloud analysis in ICA with auto-launch; compatible with analysis on local DRAGEN sever</td><td><a href="https://25033470-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FG9szlFZupV6Q2DasL98y%2Fuploads%2Fgit-blob-fe13cc9a39624ec83702316e4a89e60d5389dcc1%2FSampleSheet-NovaSeqX-Local.csv?alt=media">SampleSheet-NovaSeqX-Local.csv</a></td></tr><tr><td></td><td></td><td>Local analysis on DRAGEN server of data from a mixed flow cell; compatible with cloud analysis in ICA without auto-launch</td><td><a href="https://25033470-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FG9szlFZupV6Q2DasL98y%2Fuploads%2Fgit-blob-774593e886f61a7cd6b2ef9ac016c330ab5bb8f3%2FSampleSheet-NovaSeqX-Local-multi.csv?alt=media">SampleSheet-NovaSeqX-Local-multi.csv</a></td></tr><tr><td></td><td></td><td>Local Analysis on DRAGEN server; compatible with cloud analysis in ICA without auto-launch</td><td><a href="https://25033470-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FG9szlFZupV6Q2DasL98y%2Fuploads%2Fgit-blob-fe13cc9a39624ec83702316e4a89e60d5389dcc1%2FSampleSheet-NovaSeqX-Local%20(1).csv?alt=media">SampleSheet-NovaSeqX-Local (1).csv</a></td></tr></tbody></table>

\*Lane numbers cannot exceed what is supported by the flow cell in use.