Templates

Description

Sample Sheet templates for the Heme pipeline for standalone DRAGEN server and ICA manual launch analysis can be found in the table below. For auto-launch compatible sample sheets, use BaseSpace Run Planner.

The Heme pipeline is compatible with several instruments and assay workflows (standard, XP), each of which have implications for the sample sheet.

Templates

Sample sheet templates contain all required fields, including index sequences in the proper orientation for all indexes from a given library prep kit. The templates are provided as a starting point for creating a sample sheet manually when launching analysis on a standalone DRAGEN server or on ICA using manual launch.

For interactive run planning or to create a sample sheet for ICA Autolaunch, use BaseSpace Run Planner to create valid sample sheets for either local or cloud analysis. To set up a run in BaseSpace run planner, refer to Sample Sheet Creation in BaseSpace Run Planner.

Users can visit the Sample Sheet guidelines section to learn additional details on required fields and values as they fill-in their sample information. Use the lookup table below to select and download the sample sheet template that matches your instrument, assay, and workflow configuration:

NovaSeq 6000Dx (RUO)	Standard or XP	Cloud analysis in ICA with auto-launch generated with the BSSH run planner; compatible with analysis on local DRAGEN sever	SampleSheet-NovaSeq6000-Cloud.csv
NovaSeq 6000	​	Local Analysis on DRAGEN server; compatible with cloud analysis in ICA without auto-launch	SampleSheet-NovaSeq6000-Local.csv
NovaSeq X	Standard or XP	Cloud analysis in ICA with auto-launch of multiple pipelines for data from a mixed flow cell; compatible with analysis on local DRAGEN sever	SampleSheet-NovaSeqX-Cloud-multi.csv
​	​	Cloud analysis in ICA with auto-launch; compatible with analysis on local DRAGEN sever	SampleSheet-NovaSeqX-Cloud.csv
​	​	Local analysis on DRAGEN server of data from a mixed flow cell; compatible with cloud analysis in ICA without auto-launch	SampleSheet-NovaSeqX-Local-multi.csv
​	​	Local Analysis on DRAGEN server; compatible with cloud analysis in ICA without auto-launch	SampleSheet-NovaSeqX-Local.csv

*Lane numbers cannot exceed what is supported by the flow cell in use.