Fractional (Raw Reads) Downsampling

DRAGEN can subsample a random, fractional percentage of reads from an input file using the fractional downsampler. You can use downsampling to subsample data sets in order to simulate different amounts of sequencing. DRAGEN randomly subsamples reads from primary analysis without any modification (e.g. no trimming, no filtering, etc.).

To enable fractional downsampling, set the --enable-fractional-down-sampler command line option to true.

Any valid sequencing data format that is compatible with the DRAGEN Host Software can be used. For more information on compatible input options, see Input Options.

Command Line Options

In addition to enabling the fractional downsampling command line option, you must set the subsample fraction to downsample. To set the subsample fraction, use --down-sampler-normal-subsample and/or --down-sampler-tumor-subsample depending on the input files.

You can also specify a seed using --down-sampler-random-seed to generate different subsamples of the input data set.

Option	Description
--enable-fractional-down-sampler	Set to true to enable fractional downsampling. The default value is false.
--down-sampler-normal-subsample	Specify the fraction of reads to keep as a subsample of normal input data. The default value is 1.0 (100%).
--down-sampler-tumor-subsample	Specify the fraction of reads to keep as a subsample of tumor input data. The default value is 1.0 (100%).
--down-sampler-random-seed	Specify the random seed for different runs of the same input data. The default value is 42.