DRAGEN Reports

DRAGEN Reports are tools for generating rich, interactive and self-contained HTML reports from DRAGEN's output files. These reports combine data from QC, trimming, mapping, variant and other DRAGEN modules to create a comprehensive summary of a multi-sample workflow, as well as more detailed reports for individual samples.

Requirements

The DRAGEN Reports tools are provided as rpm packages. To use it, you will need the following:

• An x64 processor

• Red Hat Linux based operating system

In addition, while DRAGEN Reports can accept files output by any DRAGEN process (such as from a local DRAGEN box, DRAGEN equiped sequencing instrument, or the Cloud), it can only work with local files. For how to access your data and transfer it to your local environment, please refer to the user documentation for the appropriate service or instrument.

DRAGEN Reports Components

DRAGEN Reports has two tools for generating different types of report files

• dragen-reports - Generates reports for a single DRAGEN workflow or pipeline and it's samples

• dragen-summary-reports - Generate reports for a NovaSeqX run, containing multiple containing multiple DRAGEN workflows

Installation

1. Download the rpm packages from the Dragen Product Files Support Site

2. Install dragen-reports by running rpm -i dragen-reports-4.4.4-1.x86_64.rpm

3. Install dragen-summary-reports by running rpm -i dragen-summary-reports-4.4.4-1.x86_64.rpm

The command line tools are installed at /usr/bin/dragen-reports and /usr/bin/dragen-summary-reports respectively. The data used by the tools are installed at /opt/dragen-reports and /opt/dragen-summary-reports/ respectively.

Dragen-reports

The dragen-reports tool produces both workflow- and sample-level reports. A workflow-level report combines data from multiple samples analyzed by DRAGEN in the same way in order to faciliate comparssions across a batch of samples. Users can navigate to a report for a specific sample by clicking on the link in the Sample column of most workflow report tables.

Sample-level reports are also generated to allowed for more detailed analysis of individual samples. For example, under the DRAGEN-FastQC tab in a workflow report we are only able to plot the mean base quality, while for individual samples we can display a box-and-whisker plot for each position, as shown below.

To navigate back from a sample report to the multi-sample summary, users can click on the "Back to Pipeline" button located in the upper-left corner of the report.

Options

The following command-line options are available from DRAGEN Reports

Option	Argument	Description
-V, --version		Output the version string of the DRAGEN-Reports executable
-v, --verbose		Emit additional debug information whiile generating the report
-m, --manifest	Filepath	Specify a manifest file describing the report to be written
-n, --run-name	String	Manually set the run-name in the output report
-d, --directory	Comma-separated list	DRAGEN output directories to generate a report from
-o, --output	Filepath	Generate output report to the specified location
-s, --samples	Comma-separated list	Generate only individual reports for the specified sample(s)
-S, --sample-output	Filepath	Generate sample report to the specified location
-f, --force		Force overwriting of any pre-existing report files
-T, --timestamp	String	Manually set the timestamps in the output reports
-h, --help		Display this help command

Manifests

The dragen-reports tool can output reports with different contents for different DRAGEN workflows, tailoring the resulting reports to create only the most relevant plots and tables. To utilize this feature, users can use the -m / --manifest option to specify a Manifest JSON file to specify the desired output reports. These files function as recipes for different reports - specifying which files to look for, which data to process, and which plots to draw. These files are all available in the DRAGEN Reports docker under the /opt/dragen-reports/manifests directory.

Manifest	Name	Pipeline	Description
germline_wgs.json	Germline Report	DragenGermline	Default manifest targetting WGS workflows and supporting most DRAGEN variant callers
somatic_wgs.json	Somatic Report	DragenSomatic	Manifest for somatic tumor-only analysis
germline_enrichment.json	Enrichment Report	DragenEnrichment	Manifest for whole exome or germline gene panel analysis
somatic_enrichment.json	Enrichment Report	DragenEnrichment	Manifest for somatic exomes or somatic gene panel analysis
rna.json	RNA Report	DragenRna	Manifest for RNA-Seq & RNA Quantification workflows
methylation.json	Methylation Report	DragenMethylation	Manifest for the DRAGEN Methylation workflow
demux.json	Demultiplex Report	Demux	Manifest for DRAGEN demultiplex processes
bcl_convert.json	BCL Convert Report	BCLConvert	Manifest for BCLConvert and library QC workflows

Sample Script

Below is a sample Bash script for running the dragen-reports command line tool, with a line-by-line explanation

1    /usr/bin/dragen-reports \
2      -f \
3      -d /data \
4      -o /output/report.html
5      -m /opt/dragen-reports/manifests/germline_wgs.json

1. Call to execute the dragen-reports command-line tool

2. --force option, to enable over-writing of any pre-existing output files

3. --directory option specifying the input data directory

4. --output option specifying the report file to output

5. --manifest option to specify the manifest file to use - in this case we are requesting a WGS Germline report

Dragen-summary-reports

The dragen-summary-reports tool produces reports for DRAGEN analysis results from a NovaSeqX instrument. These reports summarize which DRAGEN workflows were run, which version of DRAGEN was used, which sample project they belonged to (if any), and how many samples and errors there were. If a workflow-level report is available for a given workflow, such as one generated by dragen-reports, that report will be linked from the Workflow column. In addition sub-reports are created for any sample projects, containing the subset of data for that specific project, and linked from the Sample Project column.

Options

The following command-line options are available from DRAGEN Summary Reports

Option	Argument	Description
-V, --version		Output the version string of the DRAGEN-Reports executable
-v, --verbose		Emit additional debug information whiile generating the report written
-n, --run-name	String	Manually set the run-name in the output report
-d, --directory	Directory path	DRAGEN output directories to generate a report from
-o, --output	Filepath	Generate output report to the specified location
-f, --force		Force overwriting of any pre-existing report files
-h, --help		Display this help command

Example Script

1    /usr/bin/dragen-summary-reports \
2      -f \
3      -d /data \
4      -o /data/AggregateReports/summary.html

1. Call to execute the dragen-summary-reports command-line tool

2. --force option, to enable over-writing of any pre-existing output files

3. --directory option specifying the input data directory

4. --output option specifying the report file to output

NOTE Due to the fact that dragen-summary-reports makes extensive use of symbolic links to prevent the duplication of large report files, the --directory and --output arguments must point to the same mounted volume