Advanced Topics

Advanced use cases

The Heme pipeline may be downloaded and installed on a local DRAGEN server. A download utility may be obtained from the Illumina download site, and the download utility will manage all the dependencies. Once the required installers are downloaded, the software may be installed by running the installers.

Order of installation

• Transfer the download directory content to a USB if required, and load the content onto the DRAGEN server

• Install the DRAGEN Application Manager, if not available

  • Install the docker engine, if not available

• Install the DRAGEN Heme WGS TO pipeline

  • Required resource files will be installed automatically

• Run the self test

Using NFS for data streaming

With the NovaSeq X 25B flow cells, the amount of data is on the order of terabytes, which may take a few hours or more to copy to the /staging folder on the local DRAGEN server. Using NFS storage directly for input and output is recommended in this case.