DRAGEN
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        • DRAGEN Solid WGS Tumor Normal Pipeline
          • Quick Start
          • Sample Sheets
            • Introduction
            • Requirements
            • Templates
          • Run Planning
            • Sample Sheet Creation in BaseSpace
            • Custom Config Support
          • DRAGEN Server App
            • Quick Start
            • Getting Started
            • Launching Analysis
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            • Advanced Topics
            • Custom Workflow
              • Custom Config Support
            • Illumina Connected Insights
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            • Advanced Topics
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              • Custom Config Support
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              • Illumina Connected Insights
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    • DRAGEN v4.3
      • Getting Started
      • DRAGEN Host Software
        • DRAGEN Secondary Analysis
      • DRAGEN Reference Support
        • Prepare a Reference Genome
      • DRAGEN DNA Pipeline
        • DNA Mapping
        • Read Trimming
        • DRAGEN FASTQC
        • Sorting and Duplicate Marking
        • Small Variant Calling
          • ROH Caller
          • B-Allele Frequency Output
          • Somatic Mode
          • Joint Analysis
          • De Novo Small Variant Filtering
          • Autogenerated MD5SUM for VCF Files
          • Force Genotyping
          • Machine Learning for Variant Calling
          • Evidence BAM
          • Mosaic Detection
          • VCF Imputation
          • Multi-Region Joint Detection
        • Copy Number Variant Calling
          • CNV Output
          • CNV with SV Support
          • Multisample CNV Calling
          • Somatic CNV Calling WGS
          • Somatic CNV Calling WES
          • Allele Specific CNV for Somatic WES CNV
        • Repeat Expansion Detection
          • De Novo Repeat Expansion Detection
        • Targeted Caller
          • CYPDB6 Caller
          • CYP2D6 Caller
          • CYP21A2 Caller
          • GBA Caller
          • HBA Caller
          • LPA Caller
          • Rh Caller
          • SMN Caller
        • Structural Variant Calling
          • Structural Variant De Novo Quality Scoring
        • VNTR Calling
        • Filter Duplicate Variants
        • Ploidy Calling
          • Ploidy Estimator
          • Ploidy Caller
        • Multi Caller
        • QC Metrics Reporting
        • HLA Typing
        • Biomarkers
          • Tumor Mutational Burden
          • Microsatellite Instability
          • Homologous Recombination Deficiency
          • BRCA Large Genomic Rearrangment
          • DRAGEN Fragmentomics
        • Downsampling
          • Fractional (Raw Reads) Downsampling
          • Effective Coverage Downsampling
        • Unique Molecular Identifiers
        • Indel Re-aligner (Beta)
        • Star Allele Caller
        • High Coverage Analysis
        • CheckFingerprint
        • Population Haplotyping (Beta)
        • DUX4 Rearrangement Caller
      • DRAGEN RNA Pipeline
        • RNA Alignment
        • Gene Fusion Detection
        • Gene Expression Quantification
        • RNA Variant Calling
        • Splice Variant Caller
      • DRAGEN Single-Cell Pipeline
        • scRNA
        • scATAC
        • Single-Cell Multiomics
      • DRAGEN Methylation Pipeline
      • DRAGEN Amplicon Pipeline
      • Explify Analysis Pipeline
        • Kmer Classifier
        • Kmer Classifier Database Builder
      • DRAGEN Recipes
        • DNA Germline Panel UMI
        • DNA Germline Panel
        • DNA Germline WES UMI
        • DNA Germline WES
        • DNA Germline WGS UMI
        • DNA Germline WGS
        • DNA Somatic Tumor-Normal Solid Panel UMI
        • DNA Somatic Tumor-Normal Solid Panel
        • DNA Somatic Tumor-Normal Solid WES UMI
        • DNA Somatic Tumor-Normal Solid WES
        • DNA Somatic Tumor-Normal Solid WGS UMI
        • DNA Somatic Tumor-Normal Solid WGS
        • DNA Somatic Tumor-Only Heme WGS
        • DNA Somatic Tumor-Only Solid Panel UMI
        • DNA Somatic Tumor-Only Solid Panel
        • DNA Somatic Tumor-Only Solid WES UMI
        • DNA Somatic Tumor-Only Solid WES
        • DNA Somatic Tumor-Only Solid WGS UMI
        • DNA Somatic Tumor-Only Solid WGS
        • DNA Somatic Tumor-Only ctDNA Panel UMI
        • RNA Panel
        • RNA WTS
      • BCL conversion
      • Illumina Connected Annotations
      • ORA Compression
      • Command Line Options
      • DRAGEN Reports
      • Tools and Utilities
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  1. Product Guides
  2. DRAGEN v4.4
  3. Clinical Research Workflows
  4. DRAGEN Solid WGS Tumor Normal Pipeline
  5. DRAGEN Server App

Quick Start

PreviousDRAGEN Server AppNextGetting Started

Last updated 2 days ago

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Start the pipeline with the DRAGEN_Solid_WGS_TO-4.4.4.sh Bash script. The script is installed in the /usr/local/bin directory. The Bash script is executed on the command line and runs the software with Docker (or Apptainer if specified).

For arguments, refer to . You can start from BCL files or from the FASTQ folder produced by BCL Convert. The following requirements apply for both methods:

  • Path to the sequencing run or FASTQ folder. Copy the run or FASTQ folder to the DRAGEN server into the staging folder with the following recommended organization: /staging/runs/{RunID}. You can copy the run folder onto the DRAGEN server using Linux commands such as rsync. The sample sheet within the run folder is used unless otherwise specified through the command line.

  • Run folder must be intact. Refer to for input requirements.

  • If the analysis output folder path is different from the default, provide the analysis output folder path. Refer to .

Before running the analysis, confirm that the output directory for the software to write to is empty and does not include results of previous analyses.

Storage Requirements

For optimal performance, run analysis on data stored locally on the DRAGEN server. Analysis of data stored on NAS can take longer and performance can be less reliable.

The DRAGEN server provides an NVMe SSD in the /staging directory to use as the software output directory. Network-attached storage is required for long-term storage.

When running the pipeline, use the default settings or set the -analysisFolder command line option to a directory in /staging to make sure the DRAGEN server processes read and write data on the NVMe SSD.

Before beginning analysis, develop a strategy to copy data from the DRAGEN server to a network‑attached storage. Delete output data on the DRAGEN server as soon as possible.

The following are the run and analysis output sizes for each sequencing system per 101 bp:

Sequencing System
Run Folder Output (Gb)
Analysis Output (Gb)
Minimum Disk Space (Gb)

NextSeq 500/550/550Dx (RUO) HO flow cell

32-55

82-85

150

NovaSeq 6000/6000Dx (RUO) SP Flow Cell

85-100

250-374

300

NovaSeq 6000/6000Dx (RUO) S1 Flow Cell

164-200

360-665

800

NovaSeq 6000/6000Dx (RUO) S2 Flow Cell

290-460

890-1600

1500

NovaSeq 6000/6000Dx (RUO) S4 Flow Cell

800-1200

2700-4100

3000

NovaSeq X 1.5B

213

352

800

NovaSeq X 10B

1100

1800

3000

NovaSeq X 25B

1800

3300

4000

NextSeq 1000/2000

41

107

150

When launching the analysis, the software checks that the minimum disk space required is available. If the minimum disk space is not available, the software shows an error message and prevents analysis from starting. If disk space is exhausted during a run, the run shows an error and stops analyzing.

Moving or modifying files during an analysis may cause the analysis to fail or provide incorrect results.

Command-Line Options
Command-Line Options
Starting from BCL Files