DRAGEN
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          • Quick Start
          • Sample Sheets
            • Introduction
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            • Sample Sheet Creation in BaseSpace
            • Custom Config Support
          • DRAGEN Server App
            • Quick Start
            • Getting Started
            • Launching Analysis
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            • Advanced Topics
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              • Custom Config Support
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            • Output
            • Advanced Topics
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              • Custom Config Support
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              • Illumina Connected Insights
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    • DRAGEN v4.3
      • Getting Started
      • DRAGEN Host Software
        • DRAGEN Secondary Analysis
      • DRAGEN Reference Support
        • Prepare a Reference Genome
      • DRAGEN DNA Pipeline
        • DNA Mapping
        • Read Trimming
        • DRAGEN FASTQC
        • Sorting and Duplicate Marking
        • Small Variant Calling
          • ROH Caller
          • B-Allele Frequency Output
          • Somatic Mode
          • Joint Analysis
          • De Novo Small Variant Filtering
          • Autogenerated MD5SUM for VCF Files
          • Force Genotyping
          • Machine Learning for Variant Calling
          • Evidence BAM
          • Mosaic Detection
          • VCF Imputation
          • Multi-Region Joint Detection
        • Copy Number Variant Calling
          • CNV Output
          • CNV with SV Support
          • Multisample CNV Calling
          • Somatic CNV Calling WGS
          • Somatic CNV Calling WES
          • Allele Specific CNV for Somatic WES CNV
        • Repeat Expansion Detection
          • De Novo Repeat Expansion Detection
        • Targeted Caller
          • CYPDB6 Caller
          • CYP2D6 Caller
          • CYP21A2 Caller
          • GBA Caller
          • HBA Caller
          • LPA Caller
          • Rh Caller
          • SMN Caller
        • Structural Variant Calling
          • Structural Variant De Novo Quality Scoring
        • VNTR Calling
        • Filter Duplicate Variants
        • Ploidy Calling
          • Ploidy Estimator
          • Ploidy Caller
        • Multi Caller
        • QC Metrics Reporting
        • HLA Typing
        • Biomarkers
          • Tumor Mutational Burden
          • Microsatellite Instability
          • Homologous Recombination Deficiency
          • BRCA Large Genomic Rearrangment
          • DRAGEN Fragmentomics
        • Downsampling
          • Fractional (Raw Reads) Downsampling
          • Effective Coverage Downsampling
        • Unique Molecular Identifiers
        • Indel Re-aligner (Beta)
        • Star Allele Caller
        • High Coverage Analysis
        • CheckFingerprint
        • Population Haplotyping (Beta)
        • DUX4 Rearrangement Caller
      • DRAGEN RNA Pipeline
        • RNA Alignment
        • Gene Fusion Detection
        • Gene Expression Quantification
        • RNA Variant Calling
        • Splice Variant Caller
      • DRAGEN Single-Cell Pipeline
        • scRNA
        • scATAC
        • Single-Cell Multiomics
      • DRAGEN Methylation Pipeline
      • DRAGEN Amplicon Pipeline
      • Explify Analysis Pipeline
        • Kmer Classifier
        • Kmer Classifier Database Builder
      • DRAGEN Recipes
        • DNA Germline Panel UMI
        • DNA Germline Panel
        • DNA Germline WES UMI
        • DNA Germline WES
        • DNA Germline WGS UMI
        • DNA Germline WGS
        • DNA Somatic Tumor-Normal Solid Panel UMI
        • DNA Somatic Tumor-Normal Solid Panel
        • DNA Somatic Tumor-Normal Solid WES UMI
        • DNA Somatic Tumor-Normal Solid WES
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        • DNA Somatic Tumor-Only Solid WGS
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  • Analysis on DRAGEN Server
  • Prerequisites
  • NFS and CIFS file servers
  • Starting from BCL Files
  • Starting from FASTQ Files
  • FASTQ File Organization

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  1. Product Guides
  2. DRAGEN v4.4
  3. Clinical Research Workflows
  4. DRAGEN Solid WGS Tumor Normal Pipeline

DRAGEN Server App

Analysis on DRAGEN Server

Prerequisites

  • DRAGEN Phase 3 or 4 server

  • DRAGEN License

  • Network Storage Sever

DRAGEN server

DRAGEN phase 4 server is recommended especially for datasets from NovaSeq X instruments. The server has 12 TB of intermediate data storage space for full processing of a NovaSeq X 25B flow cell.

DRAGEN phase 3 server has 6 TB of intermediate data storage space, and is recommended for flow cells from the NovaSeq 6000 or 6000 Dx instruments.

DRAGEN license

The pipeline uses the standard DRAGEN license without requiring any special licenses.

NFS and CIFS file servers

The pipeline is designed to stream data from a network file server onto the DRAGEN server, complete the analysis using the /staging area of the high performance SSD. and then stream the analysis output back to the network file server.

The network file server may be mounted to the DRAGEN server using the NFS or CIFS protocol.

Starting from BCL Files

If starting from BCL (*.bcl) files, the pipeline requires the run folder to contain certain files and folders. These inputs are required for Docker.

The run folder contains data from the sequencing run, make sure that the folder contains the following files:

Folder/File
Description

Config folder

Configuration files

Data folder

*.bcl files

Images folder

[Optional] Raw sequencing image files.

Interop folder

Interop metric files.

Logs folder

[Optional] Sequencing system log files.

RTALogs folder

Real-Time Analysis (RTA) log files.

RunInfo.xml file

Run information.

RunParameters.xml file

Run parameters.

SampleSheet.csv file

Sample information. If you want to use a sample sheet that is not in the run folder or a sample sheet named something other than SampleSheet.csv, provide the full path.

Starting from FASTQ Files

The following inputs are required for running the using FASTQ (*.fastq) files. The requirements apply to Docker.

  • Full path to an existing FASTQ folder.

  • The sample sheet is in the FASTQ folder path, or you can set the path to the sample sheet with the --sampleSheet override command line option.

Make sure there is sufficient disk space for the analysis to complete. Refer to the --help command line argument details for disk space requirements.

Use BCL Convert to produce FASTQ files for the pipeline. Using bcl2fastq does not produce the same results and is discouraged.Make sure that BCL Convert is set to write UMI sequences to the read headers in the FASTQ files.

FASTQ File Organization

Store FASTQ files in individual subfolders that correspond to a specific Sample_ID. Keep file pairs together in the same folder. Alternatively, store the FASTQ files in one flat folder structure where the FASTQ files are stored in one folder.

The pipeline requires separate FASTQ files per sample. Do not merge FASTQ files.

The instrument generates two FASTQ files per flow cell lane, so that there are eight FASTQ files per sample.

Sample1_S1_L001_R1_001.fastq.gz

  • Sample1 represents the Sample ID.

  • The S in S1 means sample, and the 1 in S1 is based on the order of samples in the sample sheet, so S1 is the first sample.

  • L001 represents the flow cell lane number.

  • The R in R1 means Read, so R1 refers to Read 1.

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The FASTQ folder structure conforms to the folder structure in

FASTQ File Organization.