DRAGEN
Illumina Connected Software
  • Overview
    • Illumina® DRAGEN™ Secondary Analysis
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    • DRAGEN v4.4
      • Getting Started
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          • Quick Start
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            • Launching Analysis
            • Advanced Topics
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          • Analysis Output
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          • Troubleshooting
        • DRAGEN Solid WGS Tumor Normal Pipeline
          • Quick Start
          • Sample Sheets
            • Introduction
            • Requirements
            • Templates
          • Run Planning
            • Sample Sheet Creation in BaseSpace
            • Custom Config Support
          • DRAGEN Server App
            • Quick Start
            • Getting Started
            • Launching Analysis
            • Command Line Options
            • Output
            • Advanced Topics
            • Custom Workflow
              • Custom Config Support
            • Illumina Connected Insights
          • ICA Cloud App
            • Getting Started
            • Launching Analysis
            • Output
            • Advanced Topics
              • Custom Workflow
              • Custom Config Support
              • Post Processing
              • Illumina Connected Insights
          • Analysis Output
          • Analysis Methods
          • Troubleshooting
      • DRAGEN Recipes
        • DNA Germline Panel UMI
        • DNA Germline Panel
        • DNA Germline WES UMI
        • DNA Germline WES
        • DNA Germline WGS UMI
        • DNA Germline WGS
        • DNA Somatic Tumor-Normal Solid Panel UMI
        • DNA Somatic Tumor-Normal Solid Panel
        • DNA Somatic Tumor-Normal Solid WES UMI
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        • DNA Somatic Tumor-Normal Solid WGS UMI
        • DNA Somatic Tumor-Normal Solid WGS
        • DNA Somatic Tumor-Only Heme WGS
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        • DNA Somatic Tumor-Only Solid Panel
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        • DNA Somatic Tumor-Only Solid WES
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        • DNA Somatic Tumor-Only Solid WGS
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        • Prepare a Reference Genome
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          • Available pipelines
            • Germline CNV Calling (WGS/WES)
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          • Additional documentation
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        • Indel Re-aligner (Beta)
        • Star Allele Caller
        • High Coverage Analysis
        • CheckFingerprint
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      • DRAGEN RNA Pipeline
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        • Splice Variant Caller
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        • Illumina PIPseq scRNA
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      • DRAGEN Amplicon Pipeline
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        • Kmer Classifier Database Builder
      • BCL conversion
      • Illumina Connected Annotations
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      • Command Line Options
        • Docker Requirements
      • DRAGEN Reports
      • Tools and Utilities
    • DRAGEN v4.3
      • Getting Started
      • DRAGEN Host Software
        • DRAGEN Secondary Analysis
      • DRAGEN Reference Support
        • Prepare a Reference Genome
      • DRAGEN DNA Pipeline
        • DNA Mapping
        • Read Trimming
        • DRAGEN FASTQC
        • Sorting and Duplicate Marking
        • Small Variant Calling
          • ROH Caller
          • B-Allele Frequency Output
          • Somatic Mode
          • Joint Analysis
          • De Novo Small Variant Filtering
          • Autogenerated MD5SUM for VCF Files
          • Force Genotyping
          • Machine Learning for Variant Calling
          • Evidence BAM
          • Mosaic Detection
          • VCF Imputation
          • Multi-Region Joint Detection
        • Copy Number Variant Calling
          • CNV Output
          • CNV with SV Support
          • Multisample CNV Calling
          • Somatic CNV Calling WGS
          • Somatic CNV Calling WES
          • Allele Specific CNV for Somatic WES CNV
        • Repeat Expansion Detection
          • De Novo Repeat Expansion Detection
        • Targeted Caller
          • CYPDB6 Caller
          • CYP2D6 Caller
          • CYP21A2 Caller
          • GBA Caller
          • HBA Caller
          • LPA Caller
          • Rh Caller
          • SMN Caller
        • Structural Variant Calling
          • Structural Variant De Novo Quality Scoring
        • VNTR Calling
        • Filter Duplicate Variants
        • Ploidy Calling
          • Ploidy Estimator
          • Ploidy Caller
        • Multi Caller
        • QC Metrics Reporting
        • HLA Typing
        • Biomarkers
          • Tumor Mutational Burden
          • Microsatellite Instability
          • Homologous Recombination Deficiency
          • BRCA Large Genomic Rearrangment
          • DRAGEN Fragmentomics
        • Downsampling
          • Fractional (Raw Reads) Downsampling
          • Effective Coverage Downsampling
        • Unique Molecular Identifiers
        • Indel Re-aligner (Beta)
        • Star Allele Caller
        • High Coverage Analysis
        • CheckFingerprint
        • Population Haplotyping (Beta)
        • DUX4 Rearrangement Caller
      • DRAGEN RNA Pipeline
        • RNA Alignment
        • Gene Fusion Detection
        • Gene Expression Quantification
        • RNA Variant Calling
        • Splice Variant Caller
      • DRAGEN Single-Cell Pipeline
        • scRNA
        • scATAC
        • Single-Cell Multiomics
      • DRAGEN Methylation Pipeline
      • DRAGEN Amplicon Pipeline
      • Explify Analysis Pipeline
        • Kmer Classifier
        • Kmer Classifier Database Builder
      • DRAGEN Recipes
        • DNA Germline Panel UMI
        • DNA Germline Panel
        • DNA Germline WES UMI
        • DNA Germline WES
        • DNA Germline WGS UMI
        • DNA Germline WGS
        • DNA Somatic Tumor-Normal Solid Panel UMI
        • DNA Somatic Tumor-Normal Solid Panel
        • DNA Somatic Tumor-Normal Solid WES UMI
        • DNA Somatic Tumor-Normal Solid WES
        • DNA Somatic Tumor-Normal Solid WGS UMI
        • DNA Somatic Tumor-Normal Solid WGS
        • DNA Somatic Tumor-Only Heme WGS
        • DNA Somatic Tumor-Only Solid Panel UMI
        • DNA Somatic Tumor-Only Solid Panel
        • DNA Somatic Tumor-Only Solid WES UMI
        • DNA Somatic Tumor-Only Solid WES
        • DNA Somatic Tumor-Only Solid WGS UMI
        • DNA Somatic Tumor-Only Solid WGS
        • DNA Somatic Tumor-Only ctDNA Panel UMI
        • RNA Panel
        • RNA WTS
      • BCL conversion
      • Illumina Connected Annotations
      • ORA Compression
      • Command Line Options
      • DRAGEN Reports
      • Tools and Utilities
  • Reference
    • DRAGEN Server
    • DRAGEN Multi-Cloud
      • DRAGEN on AWS
      • DRAGEN on AWS Batch
      • DRAGEN on Microsoft Azure
        • Run DRAGEN VM on Azure
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        • Azure Batch Run Modes
    • DRAGEN Licensing
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    • Support
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      • Noise Baselines
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    • Release Notes
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  • Overview
  • Run on DRAGEN Server
  • Common use cases

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  1. Product Guides
  2. DRAGEN v4.4
  3. Clinical Research Workflows
  4. DRAGEN Solid WGS Tumor Normal Pipeline
  5. DRAGEN Server App

Launching Analysis

Overview

Run on DRAGEN Server

The pipeline is launched with the bash script called run_DRAGEN_Solid_WGS_TN_4.4.4.sh, which is installed in the /usr/local/bin directory. The bash script is executed on the command line and runs the software with Docker or Apptainer (formerly Singularity). For command-line options, refer to Command Line Options page in user guide for details.

By default, analysis output is written to the /staging/DRAGEN_Solid_WGS_TN_4.4.4_Analysis_ directory. To write to a different output directory, run the bash script with the following argument

  --analysisFolder <FULL_PATH_TO_ANALYSIS_FOLDER>

Do not move files when the analysis is running. Moving files during the analysis can cause the analysis to fail or provide incorrect results.

An analysis can start from a sequencing system run folder containing BCL files or from one or more input folders containing FASTQ, BAM or CRAM files. A sample sheet value is also a valid argument. The following requirements apply to each:

If launching an analysis from a run folder, specify the following argument and path:

  --runFolder <FULL_PATH_TO_RUN_FOLDER>

By default, the software uses the SampleSheet.csv in the provided path.

If launching an analysis from one or more input folders, specify the following argument and path:

  --inputFolders <COMMA_SEPARATED_FULL_PATH_TO_INPUT_FOLDERS> --inputType [BAM|CRAM|FASTQ]

By default, the software uses the SampleSheet.csv in the provided path.

The default sample sheet can be overwritten by providing the argument:

  --sampleSheet <FULL_PATH_TO_A_SAMPLE_SHEET_FILE>

Common use cases

  1. Wait for any running pipeline containers to complete before launching a new analysis. Run the following command to generate a list of running containers:docker ps

  2. Select from one of the following options:

  • Start from BCL files in the run folder with the sample sheet included in the run folder. DRAGEN_Solid_WGS_TN_4.4.4.sh \ --runFolder /staging/{RunFolderName} \ --analysisFolder /staging/{AnalysisFolderName}

  • Start from BCL files in the run folder with the sample sheet located in a folder other than the run folder. DRAGEN_Solid_WGS_TN_4.4.4.sh \ --runFolder /staging/{RunFolderName} \ --analysisFolder /staging/{AnalysisFolderName} \ --sampleSheet /staging/{SampleSheetName}.csv

  • Start from BCL files in the run folder with a different sample sheet and demultiplexing only. DRAGEN_Solid_WGS_TN_4.4.4.sh \ --runFolder /staging/{RunFolderName} \ --analysisFolder /staging/{AnalysisFolderName} \ --sampleSheet /staging/{SampleSheetName}.csv \ --demultiplexOnly

  • Start from FASTQ with the sample sheet included in the FASTQ folder and with different resources and hash table folders. DRAGEN_Solid_WGS_TN_4.4.4.sh \ --resourcesFolder /staging/illumina/DRAGEN_TSO500/resources \ --hashtableFolder /staging/illumina/DRAGEN_TSO500/ref_hashtable \ --fastqFolder /staging/{FastqFolderName} \ --analysisFolder /staging/{AnalysisFolderName}

  • Start from FASTQ folder with sample sheet included in the FASTQ folder and subset of samples or pairs. DRAGEN_Solid_WGS_TN_4.4.4.sh \ --fastqFolder /staging/{FastqFolderName} \ --analysisFolder /staging/{AnalysisFolderName} \ --sampleOrPairIDs "Pair_1,Pair2"

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