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          • ROH Caller
          • B-Allele Frequency Output
          • Somatic Mode
          • Joint Analysis
          • De Novo Small Variant Filtering
          • Autogenerated MD5SUM for VCF Files
          • Force Genotyping
          • Machine Learning for Variant Calling
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          • Mosaic Detection
          • VCF Imputation
          • Multi-Region Joint Detection
        • Copy Number Variant Calling
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          • De Novo Repeat Expansion Detection
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        • Multi Caller
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        • DUX4 Rearrangement Caller
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  • Description
  • Sample Sheet Templates
  • NovaSeq 6000Dx (RUO) Cloud
  • NovaSeq 6000Dx (RUO) Local
  • NovaSeqX Cloud (Mixed Flow Cell)
  • NovaSeqX Cloud
  • NovaSeqX Local (Mixed Flow Cell)
  • NovaSeqX Local

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  1. Product Guides
  2. DRAGEN v4.4
  3. Clinical Research Workflows
  4. DRAGEN Heme WGS Tumor Only Pipeline
  5. Sample Sheets

Templates

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Last updated 2 days ago

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Description

Sample Sheet templates for the Heme pipeline for standalone DRAGEN server and ICA manual launch analysis can be found in the table below. For auto-launch compatible sample sheets, use .

Sample Sheet Templates

The Heme pipeline is compatible with several instruments and assay workflows (standard, XP), each of which have implications for the sample sheet.

Sample sheet templates contain all required fields, including index sequences in the proper orientation for all indexes from a given library prep kit. The templates are provided as a starting point for creating a sample sheet manually when launching analysis on a standalone DRAGEN server or on ICA using manual launch.

For interactive run planning or to create a sample sheet for ICA Autolaunch, use to create valid sample sheets for either local or cloud analysis. To set up a run in BaseSpace run planner, refer to .

Users can visit the section to learn additional details on required fields and values as they fill-in their sample information. Use the lookup table below to find and copy the sample sheet template that matches your instrument, assay, and workflow configuration:

Instrument
Workflow
Template

NovaSeq 6000Dx (RUO)

Standard or XP

NovaSeq 6000

NovaSeq X

Standard or XP

*Lane numbers cannot exceed what is supported by the flow cell in use.

NovaSeq 6000Dx (RUO) Cloud

[Header],
FileFormatVersion,2
RunName,test-generate-samplesheet-for-bssh-novaseq6000
InstrumentPlatform,NovaSeq
IndexOrientation,Forward
AnalysisLocation,Cloud

[Reads]
Read1Cycles,151
Read2Cycles,151
Index1Cycles,10
Index2Cycles,10

[Sequencing_Settings]
LibraryPrepKits,IlluminaDNAPrep

[BCLConvert_Settings]
SoftwareVersion,4.4.4
AdapterRead1,CTGTCTCTTATACACATCT
AdapterRead2,CTGTCTCTTATACACATCT
RunInfoIndex2ReverseComplement,Y
Index2ColumnReverseComplement,N

[BCLConvert_Data]
Lane,Sample_ID,Index,Index2
1,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
2,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
3,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
4,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
1,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
2,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
3,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
4,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
1,Validation_Sample-59,CCTCTACATG,GATACCTCCT
2,Validation_Sample-59,CCTCTACATG,GATACCTCCT
3,Validation_Sample-59,CCTCTACATG,GATACCTCCT
4,Validation_Sample-59,CCTCTACATG,GATACCTCCT
1,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
2,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
3,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
4,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT

[Cloud_Heme_Settings]
SoftwareVersion,4.4.4
StartsFromFastq,false
StageAllSettings,true
referenceGenome,hg38
oraCompressionEnabled,true
enable_map_align,true
vc_output_evidence_bam,false
qc_detect_contamination,true
aligner_clip_pe_overhang,0

[Cloud_Heme_Data]
Sample_ID,Sample_Type,Index_ID,Index,Index2
Validation_Sample-57,DNA,UDP0085,TGGTACCTAA,CATGAGTACT
Validation_Sample-58,DNA,UDP0086,TTGGAATTCC,ACGTCAATAC
Validation_Sample-59,DNA,UDP0087,CCTCTACATG,GATACCTCCT
Validation_Sample-60,DNA,UDP0088,GGAGCGTGTA,ATCCGTAAGT

[Cloud_Settings]
GeneratedVersion,1.19.0.202504151943
Cloud_Workflow,ica_workflow_1
BCLConvert_Pipeline,urn:ilmn:ica:pipeline:1548d0ff-9e0d-46cd-ae35-ac025a276381#BCL_Convert_4_4_4-e20415
Cloud_HemeWGSTO_Pipeline,urn:ilmn:ica:pipeline:9b68ef56-7068-4fca-96fe-c668ad224540#Heme_WGS_TO_v4_4_4_32_F1_Only

[Cloud_Data]
Sample_ID,ProjectName,LibraryName,LibraryPrepKitName,IndexAdapterKitName
Validation_Sample-57,,Validation_Sample-57_TGGTACCTAA_CATGAGTACT,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
Validation_Sample-58,,Validation_Sample-58_TTGGAATTCC_ACGTCAATAC,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
Validation_Sample-59,,Validation_Sample-59_CCTCTACATG_GATACCTCCT,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
Validation_Sample-60,,Validation_Sample-60_GGAGCGTGTA_ATCCGTAAGT,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes

NovaSeq 6000Dx (RUO) Local

[Header],
FileFormatVersion,2
RunName,test-generate-samplesheet-for-local-novaseq6000
InstrumentPlatform,NovaSeq
IndexOrientation,Forward
AnalysisLocation,Local

[Reads]
Read1Cycles,151
Read2Cycles,151
Index1Cycles,10
Index2Cycles,10

[Sequencing_Settings]
LibraryPrepKits,IlluminaDNAPrep

[BCLConvert_Settings]
SoftwareVersion,4.4.4
AdapterRead1,CTGTCTCTTATACACATCT
AdapterRead2,CTGTCTCTTATACACATCT
RunInfoIndex2ReverseComplement,Y
Index2ColumnReverseComplement,N

[BCLConvert_Data]
Lane,Sample_ID,Index,Index2
1,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
2,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
3,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
4,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
1,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
2,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
3,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
4,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
1,Validation_Sample-59,CCTCTACATG,GATACCTCCT
2,Validation_Sample-59,CCTCTACATG,GATACCTCCT
3,Validation_Sample-59,CCTCTACATG,GATACCTCCT
4,Validation_Sample-59,CCTCTACATG,GATACCTCCT
1,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
2,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
3,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
4,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT

[Heme_Settings]
SoftwareVersion,4.4.4
StartsFromFastq,false
StageAllSettings,true
referenceGenome,hg38
oraCompressionEnabled,true
enable_map_align,true
vc_output_evidence_bam,false
qc_detect_contamination,true
aligner_clip_pe_overhang,0

[Heme_Data]
Sample_ID,Sample_Type,Index_ID,Index,Index2
Validation_Sample-57,DNA,UDP0085,TGGTACCTAA,CATGAGTACT
Validation_Sample-58,DNA,UDP0086,TTGGAATTCC,ACGTCAATAC
Validation_Sample-59,DNA,UDP0087,CCTCTACATG,GATACCTCCT
Validation_Sample-60,DNA,UDP0088,GGAGCGTGTA,ATCCGTAAGT

[Cloud_Settings]
GeneratedVersion,1.19.0.202504151943

[Cloud_Data]
Sample_ID,ProjectName,LibraryName,LibraryPrepKitName,IndexAdapterKitName
Validation_Sample-57,,Validation_Sample-57_TGGTACCTAA_CATGAGTACT,IlluminaDNAPrep,IDTIlmnDNARNAUDISetABCDTagmentation
Validation_Sample-58,,Validation_Sample-58_TTGGAATTCC_ACGTCAATAC,IlluminaDNAPrep,IDTIlmnDNARNAUDISetABCDTagmentation
Validation_Sample-59,,Validation_Sample-59_CCTCTACATG_GATACCTCCT,IlluminaDNAPrep,IDTIlmnDNARNAUDISetABCDTagmentation
Validation_Sample-60,,Validation_Sample-60_GGAGCGTGTA_ATCCGTAAGT,IlluminaDNAPrep,IDTIlmnDNARNAUDISetABCDTagmentation

NovaSeqX Cloud (Mixed Flow Cell)

[Header],
FileFormatVersion,2
RunName,test-samplesheet-novaseqx-bssh
InstrumentPlatform,NovaSeqXSeries
IndexOrientation,Forward
AnalysisLocation,Cloud

[Reads]
Read1Cycles,151
Read2Cycles,151
Index1Cycles,10
Index2Cycles,10

[Sequencing_Settings]
LibraryPrepKits,IlluminaDNAPrep;IlluminaDNAPCRFree

[BCLConvert_Settings]
SoftwareVersion,4.4.4
AdapterRead1,CTGTCTCTTATACACATCT
AdapterRead2,CTGTCTCTTATACACATCT
OverrideCycles,Y151;I10;I10;Y151
FastqCompressionFormat,gzip

[BCLConvert_Data]
Lane,Sample_ID,Index,Index2
1,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
2,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
3,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
4,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
1,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
2,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
3,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
4,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
1,Validation_Sample-59,CCTCTACATG,GATACCTCCT
2,Validation_Sample-59,CCTCTACATG,GATACCTCCT
3,Validation_Sample-59,CCTCTACATG,GATACCTCCT
4,Validation_Sample-59,CCTCTACATG,GATACCTCCT
1,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
2,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
3,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
4,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
5,sample05,TCCATTGCCG,GAATGCACGA

[Cloud_Heme_Settings]
SoftwareVersion,4.4.4
StartsFromFastq,false
StageAllSettings,true
referenceGenome,hg38
oraCompressionEnabled,true
enable_map_align,true
vc_output_evidence_bam,false
qc_detect_contamination,true
aligner_clip_pe_overhang,0

[Cloud_Heme_Data]
Sample_ID,Sample_Type,Index_ID,Index,Index2
Validation_Sample-57,DNA,UDP0085,TGGTACCTAA,CATGAGTACT
Validation_Sample-58,DNA,UDP0086,TTGGAATTCC,ACGTCAATAC
Validation_Sample-59,DNA,UDP0087,CCTCTACATG,GATACCTCCT
Validation_Sample-60,DNA,UDP0088,GGAGCGTGTA,ATCCGTAAGT

[Cloud_DragenGermline_Settings]
SoftwareVersion,4.3.16
MapAlignOutFormat,cram

[Cloud_DragenGermline_Data]
Sample_ID,ReferenceGenomeDir,VariantCallingMode
sample05,urn:ilmn:ica:region:82e2c412-afa0-4e5e-8dc7-c21fa9d04ba8:data:fil.686fca526b6a4df6a25008dcc1f46403#/genomes/v10/A_Thaliana-rna-10-r4.0-2.tar.gz,AllVariantCallers

[Cloud_Settings]
GeneratedVersion,1.19.0.202504151943
Cloud_Workflow,ica_workflow_1
BCLConvert_Pipeline,urn:ilmn:ica:pipeline:1548d0ff-9e0d-46cd-ae35-ac025a276381#BCL_Convert_4_4_4-e20415
Cloud_HemeWGSTO_Pipeline,urn:ilmn:ica:pipeline:9b68ef56-7068-4fca-96fe-c668ad224540#Heme_WGS_TO_v4_4_4_32_F1_Only
Cloud_DragenGermline_Pipeline,urn:ilmn:ica:pipeline:a710636d-d001-4015-85e0-d7b0e3642fa1#DRAGEN_Germline_v4_3_16_-_Sequencer_Integration_Only

[Cloud_Data]
Sample_ID,ProjectName,LibraryName,LibraryPrepKitName,IndexAdapterKitName
Validation_Sample-57,,Validation_Sample-57_TGGTACCTAA_CATGAGTACT,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
Validation_Sample-58,,Validation_Sample-58_TTGGAATTCC_ACGTCAATAC,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
Validation_Sample-59,,Validation_Sample-59_CCTCTACATG_GATACCTCCT,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
Validation_Sample-60,,Validation_Sample-60_GGAGCGTGTA_ATCCGTAAGT,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
sample05,,sample05_TCCATTGCCG_GAATGCACGA,IlluminaDNAPCRFree,IDTIlmnDNARNAUDISetABCDTagmentation

NovaSeqX Cloud

[Header],
FileFormatVersion,2
RunName,test-samplesheet-novaseqx-bssh
InstrumentPlatform,NovaSeqXSeries
IndexOrientation,Forward
AnalysisLocation,Cloud

[Reads]
Read1Cycles,151
Read2Cycles,151
Index1Cycles,10
Index2Cycles,10

[Sequencing_Settings]
LibraryPrepKits,IlluminaDNAPrep

[BCLConvert_Settings]
SoftwareVersion,4.4.4
AdapterRead1,CTGTCTCTTATACACATCT
AdapterRead2,CTGTCTCTTATACACATCT
OverrideCycles,Y151;I10;I10;Y151
FastqCompressionFormat,gzip

[BCLConvert_Data]
Lane,Sample_ID,Index,Index2
1,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
2,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
3,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
4,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
1,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
2,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
3,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
4,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
1,Validation_Sample-59,CCTCTACATG,GATACCTCCT
2,Validation_Sample-59,CCTCTACATG,GATACCTCCT
3,Validation_Sample-59,CCTCTACATG,GATACCTCCT
4,Validation_Sample-59,CCTCTACATG,GATACCTCCT
1,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
2,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
3,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
4,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT

[Cloud_Heme_Settings]
SoftwareVersion,4.4.4
StartsFromFastq,false
StageAllSettings,true
referenceGenome,hg38
oraCompressionEnabled,true
enable_map_align,true
vc_output_evidence_bam,false
qc_detect_contamination,true
aligner_clip_pe_overhang,0

[Cloud_Heme_Data]
Sample_ID,Sample_Type,Index_ID,Index,Index2
Validation_Sample-57,DNA,UDP0085,TGGTACCTAA,CATGAGTACT
Validation_Sample-58,DNA,UDP0086,TTGGAATTCC,ACGTCAATAC
Validation_Sample-59,DNA,UDP0087,CCTCTACATG,GATACCTCCT
Validation_Sample-60,DNA,UDP0088,GGAGCGTGTA,ATCCGTAAGT

[Cloud_Settings]
GeneratedVersion,1.19.0.202504151943
Cloud_Workflow,ica_workflow_1
BCLConvert_Pipeline,urn:ilmn:ica:pipeline:1548d0ff-9e0d-46cd-ae35-ac025a276381#BCL_Convert_4_4_4-e20415
Cloud_HemeWGSTO_Pipeline,urn:ilmn:ica:pipeline:9b68ef56-7068-4fca-96fe-c668ad224540#Heme_WGS_TO_v4_4_4_32_F1_Only

[Cloud_Data]
Sample_ID,ProjectName,LibraryName,LibraryPrepKitName,IndexAdapterKitName
Validation_Sample-57,,Validation_Sample-57_TGGTACCTAA_CATGAGTACT,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
Validation_Sample-58,,Validation_Sample-58_TTGGAATTCC_ACGTCAATAC,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
Validation_Sample-59,,Validation_Sample-59_CCTCTACATG_GATACCTCCT,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
Validation_Sample-60,,Validation_Sample-60_GGAGCGTGTA_ATCCGTAAGT,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes

NovaSeqX Local (Mixed Flow Cell)

[Header],
FileFormatVersion,2
RunName,test-samplesheet-novaseqx-local
InstrumentPlatform,NovaSeqXSeries
IndexOrientation,Forward
AnalysisLocation,Local

[Reads]
Read1Cycles,151
Read2Cycles,151
Index1Cycles,10
Index2Cycles,10

[Sequencing_Settings]
LibraryPrepKits,IlluminaDNAPrep;IlluminaDNAPCRFree

[BCLConvert_Settings]
SoftwareVersion,4.4.4
AdapterRead1,CTGTCTCTTATACACATCT
AdapterRead2,CTGTCTCTTATACACATCT
OverrideCycles,Y151;I10;I10;Y151
FastqCompressionFormat,gzip

[BCLConvert_Data]
Lane,Sample_ID,Index,Index2
1,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
2,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
3,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
4,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
1,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
2,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
3,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
4,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
1,Validation_Sample-59,CCTCTACATG,GATACCTCCT
2,Validation_Sample-59,CCTCTACATG,GATACCTCCT
3,Validation_Sample-59,CCTCTACATG,GATACCTCCT
4,Validation_Sample-59,CCTCTACATG,GATACCTCCT
1,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
2,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
3,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
4,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
5,sample05,TCCATTGCCG,GAATGCACGA

[Heme_Settings]
SoftwareVersion,4.4.4
StartsFromFastq,false
StageAllSettings,true
referenceGenome,hg38
oraCompressionEnabled,true
enable_map_align,true
vc_output_evidence_bam,false
qc_detect_contamination,true
aligner_clip_pe_overhang,0

[Heme_Data]
Sample_ID,Sample_Type,Index_ID,Index,Index2
Validation_Sample-57,DNA,UDP0085,TGGTACCTAA,CATGAGTACT
Validation_Sample-58,DNA,UDP0086,TTGGAATTCC,ACGTCAATAC
Validation_Sample-59,DNA,UDP0087,CCTCTACATG,GATACCTCCT
Validation_Sample-60,DNA,UDP0088,GGAGCGTGTA,ATCCGTAAGT

[DragenGermline_Settings]
SoftwareVersion,4.3.16
MapAlignOutFormat,cram
KeepFastq,false

[DragenGermline_Data]
Sample_ID,ReferenceGenomeDir,VariantCallingMode
sample05,A_Thaliana-rna-10-r4.0-2,None

[Cloud_Settings]
GeneratedVersion,1.19.0.202504151943

[Cloud_Data]
Sample_ID,ProjectName,LibraryName,LibraryPrepKitName,IndexAdapterKitName
Validation_Sample-57,,Validation_Sample-57_TGGTACCTAA_CATGAGTACT,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
Validation_Sample-58,,Validation_Sample-58_TTGGAATTCC_ACGTCAATAC,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
Validation_Sample-59,,Validation_Sample-59_CCTCTACATG_GATACCTCCT,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
Validation_Sample-60,,Validation_Sample-60_GGAGCGTGTA_ATCCGTAAGT,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
sample05,,sample05_TCCATTGCCG_GAATGCACGA,IlluminaDNAPCRFree,IDTIlmnDNARNAUDISetABCDTagmentation

NovaSeqX Local

[Header],
FileFormatVersion,2
RunName,test-samplesheet-novaseqx-local
InstrumentPlatform,NovaSeqXSeries
IndexOrientation,Forward
AnalysisLocation,Local

[Reads]
Read1Cycles,151
Read2Cycles,151
Index1Cycles,10
Index2Cycles,10

[Sequencing_Settings]
LibraryPrepKits,IlluminaDNAPrep

[BCLConvert_Settings]
SoftwareVersion,4.4.4
AdapterRead1,CTGTCTCTTATACACATCT
AdapterRead2,CTGTCTCTTATACACATCT
OverrideCycles,Y151;I10;I10;Y151
FastqCompressionFormat,gzip

[BCLConvert_Data]
Lane,Sample_ID,Index,Index2
1,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
2,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
3,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
4,Validation_Sample-57,TGGTACCTAA,CATGAGTACT
1,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
2,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
3,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
4,Validation_Sample-58,TTGGAATTCC,ACGTCAATAC
1,Validation_Sample-59,CCTCTACATG,GATACCTCCT
2,Validation_Sample-59,CCTCTACATG,GATACCTCCT
3,Validation_Sample-59,CCTCTACATG,GATACCTCCT
4,Validation_Sample-59,CCTCTACATG,GATACCTCCT
1,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
2,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
3,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT
4,Validation_Sample-60,GGAGCGTGTA,ATCCGTAAGT

[Heme_Settings]
SoftwareVersion,4.4.4
StartsFromFastq,false
StageAllSettings,true
referenceGenome,hg38
oraCompressionEnabled,true
enable_map_align,true
vc_output_evidence_bam,false
qc_detect_contamination,true
aligner_clip_pe_overhang,0

[Heme_Data]
Sample_ID,Sample_Type,Index_ID,Index,Index2
Validation_Sample-57,DNA,UDP0085,TGGTACCTAA,CATGAGTACT
Validation_Sample-58,DNA,UDP0086,TTGGAATTCC,ACGTCAATAC
Validation_Sample-59,DNA,UDP0087,CCTCTACATG,GATACCTCCT
Validation_Sample-60,DNA,UDP0088,GGAGCGTGTA,ATCCGTAAGT

[Cloud_Settings]
GeneratedVersion,1.19.0.202504151943

[Cloud_Data]
Sample_ID,ProjectName,LibraryName,LibraryPrepKitName,IndexAdapterKitName
Validation_Sample-57,,Validation_Sample-57_TGGTACCTAA_CATGAGTACT,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
Validation_Sample-58,,Validation_Sample-58_TTGGAATTCC_ACGTCAATAC,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
Validation_Sample-59,,Validation_Sample-59_CCTCTACATG_GATACCTCCT,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes
Validation_Sample-60,,Validation_Sample-60_GGAGCGTGTA_ATCCGTAAGT,IlluminaDNAPrep,IDTNexteraDnaUDIndexesSetABCD384Indexes

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BaseSpace Run Planner
BaseSpace Run Planner
Sample Sheet Creation in BaseSpace Run Planner
Sample Sheet guidelines
Cloud
Local
Cloud Mixed Flow Cell
Cloud
Local Mixed Flow Cell
Local