Introduction
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A sample sheet is required for each analysis with the pipeline. A sample sheet is a comma-separated value (*.csv) file format used by Illumina instruments, platforms, and analysis pipelines to store settings and data for sequencing and analysis. The pipeline is compatible with the sample sheet v2. For general information on the sample sheet v2, refer to .
The sample sheet includes a list of samples and their index sequences, along with additional information required to run the pipeline. For example, the Library Prep Kit is a required field in the sample sheet. Both Illumina library prep kits or custom library prep kits are supported.
When running analysis on a standalone DRAGEN server or on ICA, a valid sample sheet can be created by:
BaseSpace Run Planner (preferred), see for details
Downloading and modifying a sample sheet template following the requirements, see for details
The Run Planning section of this guide includes specific instructions to plan a run and set up a valid sample sheet for the Pipeline.